Qiagen 12888-50 土壤DNA提取试剂盒（50T）

用于从土壤中的所有生物包括G⁺细菌、酵母菌、真菌、藻类、线虫类甚至真细菌的孢子、芽孢中提取基因组DNA。而且它还可以提取其他环境样品的DNA，如沉积物、排泄物、废水、雪水等。只需500mg的样品即可得到足够的DNA。

Figure 1: Isolation of high-quality genomic DNA.

The DNeasy PowerSoil Kit was used to isolate total genomic DNA from nine different sample types. The results were compared with those from soil DNA isolation kits from two other suppliers. All manufacturer’s protocols were followed. 15 µl of eluted genomic DNA was used per lane of a 0.8% TAE agarose gel.

Figure 2: Reliable PCR analysis with the DNeasy PowerSoil...

The undiluted DNA elute (1 µl) described in Figure 1 underwent PCR analysis with eubacterial primers. The results are displayed on an 0.8% TAE agarose gel. The DNeasy PowerSoil Kit was the only kit that yielded a PCR product for landfill samples (lanes 1 and 2), lake sediment (lane 6) and mulch topsoil (lane 9). High remaining humic acid content inhibited amplification for DNA isolated with kits from other manufacturers. DNA isolated with the DNeasy PowerSoil Kit had positive amplification in 100% of samples. Other supplier kits had positive amplification in 44% of samples.