Qiagen 12143 质粒中提试剂盒

纯化至多10 mg转染级质粒或柯斯质粒DNA

• 纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度

• 高产量质粒DNA

• 经济的制备方法

• 使用LyseBlue，获得最佳的裂解效果和最大的DNA产量

QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多10 mg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。

QIAGEN Plasmid Kits纯化质粒DNA适合多种应用，如：

转染克隆

PCR

体外转录

Procedure

With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation. 

Applications

Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications, such as:

• Transfection

• Cloning

• PCR

• In vitro transcription 

Specifications