山羊抗兔（二抗）

应用

建议稀释比

免疫印迹 (WB)

1:10,000-1:200,000

免疫组化（石蜡） (IHC (P))

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

1:500-1:5,000

产品规格

种属反应

Rabbit

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Secondary Antibody

抗原

Purified Rabbit IgG, whole molecule

偶联物

HRP

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Product # 31460 has been successfully used in Western blot, IHC, ELISA and IP applications.

Product # 31460 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock™ Blocking Buffers.

Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Reconstitute with 2.0 mL of distilled water (0.8 mg/mL after restoration).

靶标信息

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

仅用于科研。不用于诊断过程。未经明确授权不得转售。