山羊抗兔IgG H&L (HRP)
参阅全部 IgG 二抗
Goat
Rabbit
适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Frmore details
Rabbit IgG, whole molecule
HRP
Liquid
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
pH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Our Abpromise guarantee covers the use of ab6721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | 1/1000. | |
WB | 1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283). Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances. | |
ELISA | 1/120000. | |
Immunomicroscopy | Use at an assay dependent concentration. | |
Dot blot | Use at an assay dependent concentration. | |
ICC | 1/1000 - 1/5000. | |
IHC-Fr | 1/1000. |
英文名 | Goat Anti-Rabbit IgG H&L (HRP) (ab6721) |
---|---|
别名 | 二抗 |
品牌 | abcam |
货号 | ab6721 |
货期 | 少量现货 |
单位 | 支 |
保存条件 | -20℃ |
浓度 | 1 mg 浓度为 2 mg/ml |
纯度 | Immunogen affinity purified |